Human Immunodeficiency Virus Type 1 (Hiv-1) P24 ELISA Kit

Concentration | O.D. | Average | Corrected ------------------------------------------ 1200 pg/ml | 2.89 | 2.987 | 2.868 3.083 600 pg/ml | 1.389 | 1.428 | 1.309 1.467 300 pg/ml | 0.755 | 0.718 | 0.599 0.68 150 pg/ml | 0.375 | 0.389 | 0.27 0.403 75 pg/ml | 0.231 | 0.246 | 0.127 0.261 37.5 pg/ml | 0.179 | 0.18 | 0.061 0.181 18.75 pg/ml | 0.145 | 0.146 | 0.027 0.146 0 pg/ml | 0.114 | 0.119 | 0 0.124

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 188 | 17.1 | 9.1 Intra-assay | 2 | 20 | 384 | 35.2 | 9.2 Intra-assay | 3 | 20 | 853 | 42.3 | 5 Inter-assay | 1 | 20 | 189 | 18 | 9.5 Inter-assay | 2 | 20 | 387 | 33.1 | 8.5 Inter-assay | 3 | 20 | 854 | 63.3 | 7.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail