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Human Nectin-2, NECTIN2 ELISA Kit

Product Specifications

Background

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

Product Name Alternative

Nectin-2

Synonyms

HVEB; PRR2; PVRL2; CD112

Gene Name

NECTIN2

Gene ID

5819

UniProt

Q92692

Target

Nectin-2

Field of Research

Immunology, Microbiology

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.212 | 2.204 | 2.094 2.195 500 pg/ml | 1.26 | 1.238 | 1.128 1.215 250 pg/ml | 0.69 | 0.707 | 0.597 0.723 125 pg/ml | 0.4 | 0.408 | 0.298 0.415 62.5 pg/ml | 0.253 | 0.268 | 0.158 0.283 31.25 pg/ml | 0.187 | 0.189 | 0.079 0.19 15.625 pg/ml | 0.156 | 0.149 | 0.039 0.141 0 pg/ml | 0.112 | 0.11 | 0 0.107

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

15.625-1000 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 137 | 8.7 | 6.4 Intra-assay | 2 | 20 | 369 | 24.1 | 6.5 Intra-assay | 3 | 20 | 786 | 38.8 | 4.9 Inter-assay | 1 | 20 | 136 | 10.3 | 7.6 Inter-assay | 2 | 20 | 367 | 23 | 6.3 Inter-assay | 3 | 20 | 793 | 57.6 | 7.3

Sensitivity

4.04 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

57742

Available Sizes

Frequently Asked Questions

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