Human Nectin-2, NECTIN2 ELISA Kit
Product Specifications
Background
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Product Name Alternative
Nectin-2
Synonyms
HVEB; PRR2; PVRL2; CD112
Gene Name
NECTIN2
Gene ID
5819
UniProt
Q92692
Target
Nectin-2
Field of Research
Immunology, Microbiology
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.212 | 2.204 | 2.094 2.195 500 pg/ml | 1.26 | 1.238 | 1.128 1.215 250 pg/ml | 0.69 | 0.707 | 0.597 0.723 125 pg/ml | 0.4 | 0.408 | 0.298 0.415 62.5 pg/ml | 0.253 | 0.268 | 0.158 0.283 31.25 pg/ml | 0.187 | 0.189 | 0.079 0.19 15.625 pg/ml | 0.156 | 0.149 | 0.039 0.141 0 pg/ml | 0.112 | 0.11 | 0 0.107
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
15.625-1000 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 137 | 8.7 | 6.4 Intra-assay | 2 | 20 | 369 | 24.1 | 6.5 Intra-assay | 3 | 20 | 786 | 38.8 | 4.9 Inter-assay | 1 | 20 | 136 | 10.3 | 7.6 Inter-assay | 2 | 20 | 367 | 23 | 6.3 Inter-assay | 3 | 20 | 793 | 57.6 | 7.3
Sensitivity
4.04 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Human
Mass
57742
Available Sizes
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