Human Vascular Endothelial Growth Factor A, VEGFA ELISA Kit

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.186 | 2.228 | 2.147 2.27 500 pg/ml | 1.167 | 1.231 | 1.15 1.295 250 pg/ml | 0.671 | 0.686 | 0.605 0.701 125 pg/ml | 0.382 | 0.395 | 0.314 0.408 62.5 pg/ml | 0.241 | 0.236 | 0.155 0.231 31.25 pg/ml | 0.172 | 0.164 | 0.083 0.156 15.625 pg/ml | 0.115 | 0.118 | 0.037 0.121 0 pg/ml | 0.083 | 0.081 | 0 0.079

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 84.7 | 2.3 | 2.8 Intra-assay | 2 | 20 | 207 | 7.6 | 3.7 Intra-assay | 3 | 20 | 712 | 36.5 | 5.1 Inter-assay | 1 | 20 | 85.5 | 6.2 | 7.2 Inter-assay | 2 | 20 | 208 | 13 | 6.2 Inter-assay | 3 | 20 | 720 | 52.8 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail