Human Activin receptor-like kinase 1, ACVRL1 ELISA Kit

Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.802 | 2.94 | 2.857 3.077 500 pg/ml | 1.59 | 1.667 | 1.584 1.744 250 pg/ml | 0.837 | 0.842 | 0.759 0.846 125 pg/ml | 0.433 | 0.461 | 0.378 0.488 62.5 pg/ml | 0.276 | 0.269 | 0.186 0.261 31.25 pg/ml | 0.177 | 0.175 | 0.092 0.172 15.625 pg/ml | 0.121 | 0.129 | 0.046 0.136 0 pg/ml | 0.083 | 0.083 | 0 0.082

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 123 | 5.3 | 4.3 Intra-assay | 2 | 20 | 263 | 8.5 | 3.2 Intra-assay | 3 | 20 | 559 | 21 | 3.8 Inter-assay | 1 | 20 | 123 | 12.9 | 10.5 Inter-assay | 2 | 20 | 262 | 17.5 | 6.7 Inter-assay | 3 | 20 | 562 | 30.8 | 5.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail