Human Endoglin, ENG ELISA Kit

Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion, , suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT) . Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 1.7 | 1.717 | 1.592 1.734 500 pg/ml | 1.048 | 1.053 | 0.928 1.058 250 pg/ml | 0.618 | 0.613 | 0.488 0.608 125 pg/ml | 0.358 | 0.36 | 0.235 0.362 62.5 pg/ml | 0.232 | 0.238 | 0.113 0.244 31.25 pg/ml | 0.16 | 0.168 | 0.043 0.176 0 pg/ml | 0.123 | 0.125 | 0 0.127

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 74.7 | 6.1 | 8.2 Intra-assay | 2 | 20 | 174 | 8.7 | 5 Intra-assay | 3 | 20 | 579 | 30.6 | 5.3 Inter-assay | 1 | 20 | 75.4 | 5.4 | 7.2 Inter-assay | 2 | 20 | 174 | 10.7 | 6.1 Inter-assay | 3 | 20 | 578 | 29.1 | 5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail