Human Neutrophil Collagenase, MMP8 ELISA Kit

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis, and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases, and tumor invasion. Neutrophil collagenase, also known as Matrix metalloproteinase-8, MMP-8, and CLG1, is a member of the peptidase M1A family. MMP-8 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 in the tumor may have a protective effect against lymph node metastasis. MMP-8 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 1.808 | 1.863 | 1.759 1.918 500 pg/ml | 1.263 | 1.2 | 1.096 1.137 250 pg/ml | 0.751 | 0.765 | 0.661 0.779 125 pg/ml | 0.435 | 0.461 | 0.357 0.487 62.5 pg/ml | 0.308 | 0.288 | 0.184 0.268 31.25 pg/ml | 0.204 | 0.196 | 0.092 0.188 15.625 pg/ml | 0.14 | 0.146 | 0.042 0.152 0 pg/ml | 0.111 | 0.104 | 0 0.097

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 88 | 3.1 | 3.5 Intra-assay | 2 | 20 | 259 | 8.1 | 3.1 Intra-assay | 3 | 20 | 740 | 40.2 | 5.4 Inter-assay | 1 | 20 | 87.4 | 7.2 | 8.3 Inter-assay | 2 | 20 | 257 | 15.9 | 6.2 Inter-assay | 3 | 20 | 744 | 52 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail