Human Serotransferrin, TF ELISA Kit

Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins that bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe3+ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe (3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.396 | 2.323 | 2.213 2.25 500 pg/ml | 1.342 | 1.336 | 1.226 1.331 250 pg/ml | 0.741 | 0.724 | 0.614 0.708 125 pg/ml | 0.462 | 0.439 | 0.329 0.416 62.5 pg/ml | 0.248 | 0.263 | 0.153 0.278 31.25 pg/ml | 0.192 | 0.193 | 0.083 0.195 15.625 pg/ml | 0.146 | 0.156 | 0.046 0.166 0 pg/ml | 0.103 | 0.11 | 0 0.117

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 87 | 2.4 | 2.7 Intra-assay | 2 | 20 | 363 | 13.3 | 3.7 Intra-assay | 3 | 20 | 755 | 20.5 | 2.7 Inter-assay | 1 | 20 | 87.1 | 3.4 | 3.9 Inter-assay | 2 | 20 | 361 | 27.3 | 7.6 Inter-assay | 3 | 20 | 751 | 27.8 | 3.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail