Human Cytotoxic T-Lymphocyte Protein 4, CTLA4 ELISA Kit

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2) . CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg) . Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets CTLA4 / CD152 Immune Checkpoint Proteins Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.272 | 2.216 | 2.118 2.159 500 pg/ml | 1.271 | 1.224 | 1.126 1.176 250 pg/ml | 0.718 | 0.673 | 0.575 0.628 125 pg/ml | 0.386 | 0.387 | 0.289 0.387 62.5 pg/ml | 0.232 | 0.247 | 0.149 0.262 31.25 pg/ml | 0.181 | 0.177 | 0.079 0.172 15.625 pg/ml | 0.137 | 0.133 | 0.035 0.129 0 pg/ml | 0.097 | 0.098 | 0 0.098

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 87.8 | 4.1 | 4.6 Intra-assay | 2 | 20 | 338 | 7.1 | 2.1 Intra-assay | 3 | 20 | 785 | 19.2 | 2.5 Inter-assay | 1 | 20 | 88.6 | 6 | 6.7 Inter-assay | 2 | 20 | 340 | 12.8 | 3.8 Inter-assay | 3 | 20 | 789 | 33.1 | 4.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail