Human Lysosome-Associated Membrane Glycoprotein 1, LAMP1 ELISA Kit

Lysosome-associated membrane glycoprotein 1, also known as CD107 antigen-like family member A, CD107a, and LAMP1, is a single-pass type I membrane protein that belongs to the LAMP family. CD107a is expressed largely in the endosome-lysosome membranes of cells but is also found on the plasma membrane (1-2% of total LAMP1) . LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD107b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in which amyloid burden was cleared following beta A-peptide immunization. Also, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide, or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 1.767 | 1.731 | 1.622 1.695 500 pg/ml | 1.118 | 1.091 | 0.982 1.064 250 pg/ml | 0.642 | 0.658 | 0.549 0.674 125 pg/ml | 0.422 | 0.432 | 0.323 0.442 62.5 pg/ml | 0.265 | 0.273 | 0.164 0.281 31.25 pg/ml | 0.201 | 0.19 | 0.081 0.179 15.625 pg/ml | 0.157 | 0.151 | 0.042 0.145 0 pg/ml | 0.105 | 0.109 | 0 0.113

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 42.7 | 1.6 | 3.8 Intra-assay | 2 | 20 | 112 | 3 | 2.7 Intra-assay | 3 | 20 | 561 | 13.7 | 2.4 Inter-assay | 1 | 20 | 43.1 | 3.4 | 7.9 Inter-assay | 2 | 20 | 112 | 7.9 | 7 Inter-assay | 3 | 20 | 565 | 41.2 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail