Human C-Reactive Protein, CRP ELISA Kit

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.419 | 2.427 | 2.329 2.436 500 pg/ml | 1.283 | 1.33 | 1.232 1.377 250 pg/ml | 0.763 | 0.732 | 0.634 0.701 125 pg/ml | 0.392 | 0.409 | 0.311 0.426 62.5 pg/ml | 0.242 | 0.236 | 0.138 0.231 31.25 pg/ml | 0.174 | 0.166 | 0.068 0.159 15.625 pg/ml | 0.119 | 0.124 | 0.026 0.129 0 pg/ml | 0.093 | 0.098 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 44 | 1.2 | 2.8 Intra-assay | 2 | 20 | 176 | 3.4 | 2 Intra-assay | 3 | 20 | 678 | 22.6 | 3.3 Inter-assay | 1 | 20 | 44.4 | 3.2 | 7.2 Inter-assay | 2 | 20 | 177 | 11.9 | 6.7 Inter-assay | 3 | 20 | 680 | 45.9 | 6.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail