Human Wap Four-Disulfide Core Domain Protein 2, WFDC2 ELISA Kit

WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein that contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.557 | 2.584 | 2.457 2.611 500 pg/ml | 1.516 | 1.487 | 1.36 1.458 250 pg/ml | 0.764 | 0.781 | 0.654 0.798 125 pg/ml | 0.442 | 0.444 | 0.317 0.446 62.5 pg/ml | 0.297 | 0.287 | 0.16 0.277 31.25 pg/ml | 0.196 | 0.209 | 0.082 0.222 15.625 pg/ml | 0.179 | 0.175 | 0.048 0.171 0 pg/ml | 0.122 | 0.127 | 0 0.132

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 97.7 | 2.6 | 2.7 Intra-assay | 2 | 20 | 250 | 8.2 | 3.3 Intra-assay | 3 | 20 | 501 | 16.8 | 3.3 Inter-assay | 1 | 20 | 98.7 | 8.3 | 8.4 Inter-assay | 2 | 20 | 249 | 17.1 | 6.9 Inter-assay | 3 | 20 | 500 | 21.3 | 4.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail