Human Pancreatic Triacylglycerol Lipase, PNLIP ELISA Kit

PNLIP is an enzyme that belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.275 | 2.172 | 2.041 2.068 500 pg/ml | 1.314 | 1.277 | 1.146 1.24 250 pg/ml | 0.732 | 0.713 | 0.582 0.694 125 pg/ml | 0.44 | 0.421 | 0.29 0.402 62.5 pg/ml | 0.255 | 0.265 | 0.134 0.274 31.25 pg/ml | 0.196 | 0.189 | 0.058 0.181 15.625 pg/ml | 0.167 | 0.159 | 0.028 0.151 0 pg/ml | 0.125 | 0.131 | 0 0.136

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 91.5 | 4.9 | 5.3 Intra-assay | 2 | 20 | 247 | 7.7 | 3.1 Intra-assay | 3 | 20 | 727 | 41.6 | 5.7 Inter-assay | 1 | 20 | 91 | 3.2 | 3.5 Inter-assay | 2 | 20 | 245 | 20 | 8.2 Inter-assay | 3 | 20 | 720 | 25 | 3.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail