Mouse Interleukin-17A, Il17a ELISA Kit

IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO) . High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.353 | 2.426 | 2.325 2.499 500 pg/ml | 1.356 | 1.333 | 1.232 1.31 250 pg/ml | 0.714 | 0.733 | 0.632 0.753 125 pg/ml | 0.408 | 0.432 | 0.331 0.456 62.5 pg/ml | 0.273 | 0.269 | 0.168 0.266 31.25 pg/ml | 0.181 | 0.185 | 0.084 0.189 15.625 pg/ml | 0.142 | 0.141 | 0.04 0.141 0 pg/ml | 0.102 | 0.101 | 0 0.101

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 139 | 6.8 | 4.9 Intra-assay | 2 | 20 | 385 | 16.1 | 4.2 Intra-assay | 3 | 20 | 786 | 67.2 | 8.6 Inter-assay | 1 | 20 | 138 | 8.6 | 6.3 Inter-assay | 2 | 20 | 382 | 28.1 | 7.4 Inter-assay | 3 | 20 | 783 | 54.7 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail