Mouse Cub Domain-Containing Protein 1, Cdcp1 ELISA Kit

CDCP1 contains three extracellular CUB domains. It is a putative stem cell marker that is highly expressed in some human cancer cells and both, typical and atypical (cancerous) colons. It interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. It also interacts with SRC and PRKCG/protein kinase C gamma. CDCP1 is taken as a key regulator of EGF/EGFR-induced cell migration. It has been shown that signaling via EGF/EGFR induces migration of ovarian cancer Caov3 and OVCA420 cells with concomitant up-regulation of CDCP1 mRNA and protein. Consistent with a role in cell migration CDCP1 relocates from cell-cell junctions to punctate structures on filopodia after activation of EGFR. It may be involved in cell adhesion and cell matrix association. It also may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. It has been taken as a novel marker for leukemia diagnosis and immature hematopoietic stem cell subsets.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.368 | 2.461 | 2.344 2.555 500 pg/ml | 1.44 | 1.403 | 1.286 1.367 250 pg/ml | 0.746 | 0.789 | 0.672 0.833 125 pg/ml | 0.468 | 0.463 | 0.346 0.459 62.5 pg/ml | 0.296 | 0.295 | 0.178 0.294 31.25 pg/ml | 0.214 | 0.212 | 0.095 0.21 15.625 pg/ml | 0.166 | 0.17 | 0.053 0.174 0 pg/ml | 0.118 | 0.117 | 0 0.117

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 121 | 6.3 | 5.2 Intra-assay | 2 | 20 | 319 | 11.1 | 3.5 Intra-assay | 3 | 20 | 694 | 18.2 | 2.6 Inter-assay | 1 | 20 | 120 | 10.6 | 8.8 Inter-assay | 2 | 20 | 317 | 32.2 | 10.2 Inter-assay | 3 | 20 | 700 | 65.3 | 9.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail