Rat Complement Component C1Q Receptor, Cd93 ELISA Kit

CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre) plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.528 | 2.551 | 2.464 2.574 500 pg/ml | 1.354 | 1.372 | 1.285 1.39 250 pg/ml | 0.694 | 0.735 | 0.648 0.776 125 pg/ml | 0.419 | 0.398 | 0.311 0.377 62.5 pg/ml | 0.234 | 0.24 | 0.153 0.245 31.25 pg/ml | 0.158 | 0.166 | 0.079 0.174 15.625 pg/ml | 0.13 | 0.124 | 0.037 0.118 0 pg/ml | 0.086 | 0.087 | 0 0.087

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 109 | 5.2 | 4.8 Intra-assay | 2 | 20 | 259 | 12.6 | 4.8 Intra-assay | 3 | 20 | 550 | 23.6 | 4.3 Inter-assay | 1 | 20 | 110 | 7.3 | 6.7 Inter-assay | 2 | 20 | 262 | 16.6 | 6.3 Inter-assay | 3 | 20 | 546 | 33.8 | 6.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail