Human Interleukin-12 Subunit Beta, IL12B ELISA Kit

Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor-like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine is important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine that functions in innate and adaptive immunity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.406 | 2.445 | 2.35 2.483 500 pg/ml | 1.384 | 1.34 | 1.245 1.295 250 pg/ml | 0.779 | 0.756 | 0.661 0.733 125 pg/ml | 0.438 | 0.441 | 0.346 0.444 62.5 pg/ml | 0.275 | 0.275 | 0.18 0.274 31.25 pg/ml | 0.171 | 0.184 | 0.089 0.196 15.625 pg/ml | 0.145 | 0.143 | 0.048 0.14 0 pg/ml | 0.09 | 0.095 | 0 0.099

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 109 | 4.2 | 3.9 Intra-assay | 2 | 20 | 261 | 7 | 2.7 Intra-assay | 3 | 20 | 621 | 14.7 | 2.4 Inter-assay | 1 | 20 | 108 | 8.4 | 7.8 Inter-assay | 2 | 20 | 259 | 18.3 | 7.1 Inter-assay | 3 | 20 | 623 | 45.6 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail