Human Alpha-2-Hs-Glycoprotein, AHSG ELISA Kit

Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

Concentration | O.D. | Average | Corrected ------------------------------------------ 900 pg/ml | 2.436 | 2.347 | 2.266 2.258 450 pg/ml | 1.391 | 1.347 | 1.266 1.302 225 pg/ml | 0.765 | 0.774 | 0.693 0.782 112.5 pg/ml | 0.418 | 0.43 | 0.349 0.441 56.25 pg/ml | 0.275 | 0.265 | 0.184 0.254 28.125 pg/ml | 0.177 | 0.172 | 0.091 0.166 14.0625 pg/ml | 0.14 | 0.138 | 0.057 0.135 0 pg/ml | 0.081 | 0.081 | 0 0.08

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 73.1 | 4.3 | 5.9 Intra-assay | 2 | 20 | 183 | 8.1 | 4.4 Intra-assay | 3 | 20 | 468 | 21.3 | 4.6 Inter-assay | 1 | 20 | 73.6 | 7.2 | 9.8 Inter-assay | 2 | 20 | 184 | 13.3 | 7.2 Inter-assay | 3 | 20 | 467 | 27.9 | 6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail