Human Interleukin-10, IL10 ELISA Kit

IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 900 pg/ml | 2.389 | 2.458 | 2.364 2.527 450 pg/ml | 1.49 | 1.42 | 1.326 1.35 225 pg/ml | 0.739 | 0.746 | 0.652 0.753 112.5 pg/ml | 0.403 | 0.396 | 0.302 0.389 56.25 pg/ml | 0.257 | 0.242 | 0.148 0.226 28.125 pg/ml | 0.154 | 0.16 | 0.066 0.166 14.0625 pg/ml | 0.122 | 0.119 | 0.025 0.115 0 pg/ml | 0.092 | 0.094 | 0 0.096

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 92 | 4.9 | 5.3 Intra-assay | 2 | 20 | 249 | 10.6 | 4.3 Intra-assay | 3 | 20 | 655 | 43.4 | 6.6 Inter-assay | 1 | 20 | 91.8 | 10 | 10.9 Inter-assay | 2 | 20 | 247 | 19.9 | 8 Inter-assay | 3 | 20 | 652 | 55.9 | 8.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail