Human Interleukin-15, IL15 ELISA Kit

The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x (L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.482 | 2.446 | 2.36 2.41 400 pg/ml | 1.332 | 1.4 | 1.314 1.467 200 pg/ml | 0.764 | 0.815 | 0.729 0.866 100 pg/ml | 0.436 | 0.449 | 0.363 0.462 50 pg/ml | 0.245 | 0.252 | 0.166 0.259 25 pg/ml | 0.166 | 0.178 | 0.092 0.19 12.5 pg/ml | 0.124 | 0.127 | 0.041 0.13 0 pg/ml | 0.092 | 0.086 | 0 0.08

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 51.2 | 2.2 | 4.3 Intra-assay | 2 | 20 | 183 | 7.3 | 4 Intra-assay | 3 | 20 | 610 | 21.9 | 3.6 Inter-assay | 1 | 20 | 51.5 | 3.7 | 7.3 Inter-assay | 2 | 20 | 184 | 11.5 | 6.3 Inter-assay | 3 | 20 | 616 | 50.2 | 8.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail