Human Carboxypeptidase A2, CPA2 ELISA Kit

Carboxypeptidase A2 (CPA2) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.859 | 2.792 | 2.693 2.724 400 pg/ml | 1.604 | 1.624 | 1.525 1.643 200 pg/ml | 0.806 | 0.856 | 0.757 0.906 100 pg/ml | 0.472 | 0.486 | 0.387 0.499 50 pg/ml | 0.276 | 0.289 | 0.19 0.301 25 pg/ml | 0.181 | 0.191 | 0.092 0.2 12.5 pg/ml | 0.151 | 0.144 | 0.045 0.136 0 pg/ml | 0.093 | 0.099 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 46.7 | 3.3 | 7.2 Intra-assay | 2 | 20 | 234 | 10.4 | 4.5 Intra-assay | 3 | 20 | 615 | 28.5 | 4.6 Inter-assay | 1 | 20 | 46.8 | 3.6 | 7.6 Inter-assay | 2 | 20 | 235 | 15.2 | 6.5 Inter-assay | 3 | 20 | 613 | 40.2 | 6.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail