Human Fibroblast Growth Factor Receptor 4, FGFR4 ELISA Kit

Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.176 | 2.169 | 2.061 2.162 400 pg/ml | 1.247 | 1.207 | 1.099 1.167 200 pg/ml | 0.692 | 0.672 | 0.564 0.652 100 pg/ml | 0.401 | 0.396 | 0.288 0.391 50 pg/ml | 0.245 | 0.253 | 0.145 0.262 25 pg/ml | 0.181 | 0.178 | 0.07 0.175 12.5 pg/ml | 0.152 | 0.144 | 0.036 0.137 0 pg/ml | 0.101 | 0.108 | 0 0.115

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 107 | 3.1 | 2.9 Intra-assay | 2 | 20 | 242 | 9.2 | 3.8 Intra-assay | 3 | 20 | 591 | 30.2 | 5.1 Inter-assay | 1 | 20 | 106 | 7.5 | 7.1 Inter-assay | 2 | 20 | 244 | 14.9 | 6.1 Inter-assay | 3 | 20 | 584 | 43.2 | 7.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail