Human Kunitz-Type Protease Inhibitor 1, SPINT1 ELISA Kit

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.115 | 2.205 | 2.086 2.294 400 pg/ml | 1.213 | 1.201 | 1.082 1.188 200 pg/ml | 0.708 | 0.665 | 0.546 0.622 100 pg/ml | 0.384 | 0.38 | 0.261 0.376 50 pg/ml | 0.246 | 0.252 | 0.133 0.257 25 pg/ml | 0.187 | 0.192 | 0.073 0.197 12.5 pg/ml | 0.171 | 0.164 | 0.045 0.157 0 pg/ml | 0.115 | 0.119 | 0 0.123

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 41 | 3.8 | 9.2 Intra-assay | 2 | 20 | 105 | 10.5 | 10 Intra-assay | 3 | 20 | 420 | 41 | 9.8 Inter-assay | 1 | 20 | 40.8 | 4.5 | 11.1 Inter-assay | 2 | 20 | 104 | 11.3 | 10.8 Inter-assay | 3 | 20 | 418 | 38.4 | 9.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail