Human Nf-Kappa-B Inhibitor Alpha, NFKBIA ELISA Kit

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. Also, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates the transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM) . The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 1.948 | 1.943 | 1.851 1.939 400 pg/ml | 1.125 | 1.07 | 0.978 1.015 200 pg/ml | 0.598 | 0.591 | 0.499 0.585 100 pg/ml | 0.358 | 0.364 | 0.272 0.371 50 pg/ml | 0.217 | 0.228 | 0.136 0.239 25 pg/ml | 0.186 | 0.197 | 0.105 0.209 12.5 pg/ml | 0.157 | 0.148 | 0.056 0.139 0 pg/ml | 0.092 | 0.092 | 0 0.093

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 91.6 | 2.6 | 2.9 Intra-assay | 2 | 20 | 191 | 7.1 | 3.7 Intra-assay | 3 | 20 | 530 | 27.3 | 5.2 Inter-assay | 1 | 20 | 91.3 | 6.5 | 7.2 Inter-assay | 2 | 20 | 190 | 11.9 | 6.3 Inter-assay | 3 | 20 | 534 | 38.5 | 7.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail