Mouse Carboxypeptidase B1 ELISA Kit

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.792 | 2.813 | 2.718 2.834 400 pg/ml | 1.616 | 1.569 | 1.474 1.522 200 pg/ml | 0.835 | 0.847 | 0.752 0.859 100 pg/ml | 0.435 | 0.467 | 0.372 0.499 50 pg/ml | 0.281 | 0.295 | 0.2 0.309 25 pg/ml | 0.18 | 0.187 | 0.092 0.194 12.5 pg/ml | 0.143 | 0.142 | 0.047 0.141 0 pg/ml | 0.091 | 0.095 | 0 0.099

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 80.4 | 1.8 | 2.3 Intra-assay | 2 | 20 | 167 | 3.9 | 2.3 Intra-assay | 3 | 20 | 419 | 13.5 | 3.2 Inter-assay | 1 | 20 | 80.1 | 6.6 | 8.2 Inter-assay | 2 | 20 | 168 | 13.6 | 8.1 Inter-assay | 3 | 20 | 418 | 30.3 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail