Mouse T-Cell Surface Glycoprotein Cd5, Cd5 ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 3.44 | 3.319 | 3.184 3.198 400 pg/ml | 1.534 | 1.607 | 1.472 1.679 200 pg/ml | 0.699 | 0.734 | 0.599 0.769 100 pg/ml | 0.369 | 0.371 | 0.236 0.372 50 pg/ml | 0.236 | 0.223 | 0.088 0.209 25 pg/ml | 0.181 | 0.175 | 0.04 0.169 12.5 pg/ml | 0.165 | 0.157 | 0.022 0.148 0 pg/ml | 0.134 | 0.135 | 0 0.136

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 94 | 5.6 | 6 Intra-assay | 2 | 20 | 209 | 7.6 | 3.6 Intra-assay | 3 | 20 | 564 | 25.9 | 4.6 Inter-assay | 1 | 20 | 94.6 | 8.9 | 9.4 Inter-assay | 2 | 20 | 209 | 10.2 | 4.9 Inter-assay | 3 | 20 | 569 | 25.9 | 4.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail