Mouse Osteomodulin, Omd ELISA Kit

Osteomodulin (OMD), also known as Osteoadherin (OSAD), Keratan sulfate proteoglycan osteomodulin, KSPG osteomodulin, and SLRR2C, is a secreted protein that belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. SLRP family proteins are normally found in extracellular matrices, but Osteomodulin is the only member restricted to mineralized tissues. Osteomodulin is primarily expressed by osteoblasts and might have a role in the regulation of mineralization. In bone, OSAD has been localized in the primary spongiosa within the bovine fetal rib growth plate. Moreover, in situ hybridization has shown expression of OSAD in osteoblasts close to the cartilage and bone border in the growth plate of rat femur. OSAD may play an important role during tooth development and biomineralization of dentin. Osteomodulin is a cell binding keratan sulfate proteoglycan that was recently isolated from mineralized bovine bone and subsequently cloned and sequenced. Osteomodulin may be implicated in biomineralization processes. It has a function in the binding of osteoblasts via the alpha (V) beta (3) -integrin. Osteomodulin is likely an osteoblast maturation marker that is induced by osteoclast activity. Osteomodulin is also an early marker for terminally differentiated matrix producing osteoblasts.

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.717 | 2.826 | 2.696 2.936 400 pg/ml | 1.486 | 1.522 | 1.392 1.559 200 pg/ml | 0.861 | 0.869 | 0.739 0.878 100 pg/ml | 0.495 | 0.501 | 0.371 0.508 50 pg/ml | 0.287 | 0.308 | 0.178 0.33 25 pg/ml | 0.223 | 0.22 | 0.09 0.218 12.5 pg/ml | 0.174 | 0.174 | 0.044 0.175 0 pg/ml | 0.129 | 0.13 | 0 0.131

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 46.3 | 2.3 | 4.9 Intra-assay | 2 | 20 | 103 | 5.1 | 5 Intra-assay | 3 | 20 | 412 | 17.6 | 4.3 Inter-assay | 1 | 20 | 46.6 | 3.1 | 6.6 Inter-assay | 2 | 20 | 103 | 6.5 | 6.4 Inter-assay | 3 | 20 | 415 | 25.3 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail