Rhesus Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii ELISA Kit

Signal transduction, Immunology bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR) . FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16) . CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Concentration | O.D. | Average | Corrected ------------------------------------------ 800 pg/ml | 2.226 | 2.248 | 2.137 2.27 400 pg/ml | 1.26 | 1.279 | 1.168 1.298 200 pg/ml | 0.742 | 0.729 | 0.618 0.716 100 pg/ml | 0.381 | 0.408 | 0.297 0.434 50 pg/ml | 0.275 | 0.27 | 0.159 0.265 25 pg/ml | 0.192 | 0.187 | 0.076 0.182 12.5 pg/ml | 0.153 | 0.149 | 0.038 0.145 0 pg/ml | 0.108 | 0.111 | 0 0.114

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 37.4 | 1.3 | 3.6 Intra-assay | 2 | 20 | 121 | 4 | 3.3 Intra-assay | 3 | 20 | 603 | 21.9 | 3.6 Inter-assay | 1 | 20 | 37.3 | 2.1 | 5.7 Inter-assay | 2 | 20 | 121 | 5.6 | 4.6 Inter-assay | 3 | 20 | 597 | 23.4 | 3.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail