Human Neurotrophic tyrosine kinase receptor type 2, NTRK2 ELISA Kit

TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptor with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K, and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB, and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has the highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, long-term potentiation, and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 700 pg/ml | 2.49 | 2.431 | 2.35 2.372 350 pg/ml | 1.456 | 1.385 | 1.304 1.314 175 pg/ml | 0.721 | 0.748 | 0.667 0.775 87.5 pg/ml | 0.432 | 0.41 | 0.329 0.388 43.75 pg/ml | 0.227 | 0.237 | 0.156 0.247 21.875 pg/ml | 0.154 | 0.148 | 0.067 0.143 10.9375 pg/ml | 0.117 | 0.111 | 0.03 0.105 0 pg/ml | 0.082 | 0.081 | 0 0.08

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 82.6 | 2.2 | 2.7 Intra-assay | 2 | 20 | 180 | 5.3 | 3 Intra-assay | 3 | 20 | 486 | 14.4 | 3 Inter-assay | 1 | 20 | 83.3 | 5.7 | 6.8 Inter-assay | 2 | 20 | 180 | 10.5 | 5.9 Inter-assay | 3 | 20 | 487 | 37.9 | 7.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail