Human Xaa-Pro Aminopeptidase 2, XPNPEP2 ELISA Kit

Aminopeptidase P (APP) is a hydrolase specific for N-terminal imido bonds, which are common to several collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. A membrane-bound and soluble form of this enzyme (XPNPEP2) have been identified as products of two separate genes. XPNPEP2, the X-linked gene that encodes membranous aminopeptidase P (APP), has been reported to associate with APP activity. The membrane aminopeptidase P (XPNPEP2) is largely limited in distribution to endothelia and brush border epithelia. APP and XPNPEP2 contain homologous blocks of sequence common to members of the "pita bread-fold" protein family, of which Escherichia coli methionine aminopeptidase is the prototype. The C-2399A variant in XPNPEP2 is associated with reduced APP activity and a higher incidence of AE-ACEi. XPNPEP2 mRNA was detected in fibroblasts that carry the translocation, suggesting that this gene at least partially escapes X inactivation. XPNPEP2 is a candidate gene for premature ovarian failure (POF) .

Concentration | O.D. | Average | Corrected ------------------------------------------ 700 pg/ml | 2.865 | 2.962 | 2.851 3.059 350 pg/ml | 1.701 | 1.624 | 1.513 1.548 175 pg/ml | 0.946 | 0.889 | 0.778 0.833 87.5 pg/ml | 0.492 | 0.522 | 0.411 0.553 43.75 pg/ml | 0.313 | 0.323 | 0.212 0.334 21.875 pg/ml | 0.229 | 0.217 | 0.106 0.206 10.9375 pg/ml | 0.164 | 0.164 | 0.053 0.165 0 pg/ml | 0.111 | 0.111 | 0 0.112

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 42.5 | 2.1 | 5 Intra-assay | 2 | 20 | 152 | 6.1 | 4 Intra-assay | 3 | 20 | 389 | 20.9 | 5.4 Inter-assay | 1 | 20 | 42.9 | 4 | 9.3 Inter-assay | 2 | 20 | 152 | 6 | 4 Inter-assay | 3 | 20 | 392 | 23.9 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail