Mouse T-Cell Differentiation Antigen Cd6, Cd6 ELISA Kit

T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6 / TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP12 binds to activated leukocyte cell adhesion molecule (CD166), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP12 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Concentration | O.D. | Average | Corrected ------------------------------------------ 700 pg/ml | 2.163 | 2.237 | 2.153 2.311 350 pg/ml | 1.197 | 1.18 | 1.096 1.164 175 pg/ml | 0.625 | 0.638 | 0.554 0.652 87.5 pg/ml | 0.345 | 0.358 | 0.274 0.372 43.75 pg/ml | 0.21 | 0.225 | 0.141 0.241 21.875 pg/ml | 0.155 | 0.148 | 0.064 0.141 10.9375 pg/ml | 0.114 | 0.12 | 0.036 0.126 0 pg/ml | 0.08 | 0.084 | 0 0.089

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 59.4 | 3.1 | 5.2 Intra-assay | 2 | 20 | 180 | 6.7 | 3.7 Intra-assay | 3 | 20 | 462 | 21.1 | 4.6 Inter-assay | 1 | 20 | 59.5 | 6 | 10 Inter-assay | 2 | 20 | 178 | 17.6 | 9.9 Inter-assay | 3 | 20 | 467 | 39.3 | 8.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail