Human Interleukin-4, IL4 ELISA Kit

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.681 | 2.755 | 2.637 2.83 250 pg/ml | 1.305 | 1.314 | 1.196 1.323 125 pg/ml | 0.713 | 0.682 | 0.564 0.651 62.5 pg/ml | 0.369 | 0.368 | 0.25 0.368 31.25 pg/ml | 0.244 | 0.236 | 0.118 0.228 15.625 pg/ml | 0.159 | 0.168 | 0.05 0.177 7.8125 pg/ml | 0.142 | 0.146 | 0.028 0.15 0 pg/ml | 0.125 | 0.118 | 0 0.111

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 75.7 | 3.6 | 4.8 Intra-assay | 2 | 20 | 168 | 6.8 | 4.1 Intra-assay | 3 | 20 | 421 | 36.6 | 8.7 Inter-assay | 1 | 20 | 75.5 | 4.9 | 6.4 Inter-assay | 2 | 20 | 169 | 12.7 | 7.5 Inter-assay | 3 | 20 | 424 | 29.9 | 7.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail