Human Cd276 Antigen, CD276 ELISA Kit

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 600 pg/ml | 2.426 | 2.489 | 2.389 2.552 300 pg/ml | 1.395 | 1.384 | 1.284 1.372 150 pg/ml | 0.744 | 0.736 | 0.636 0.727 75 pg/ml | 0.439 | 0.414 | 0.314 0.389 37.5 pg/ml | 0.254 | 0.252 | 0.152 0.25 18.75 pg/ml | 0.167 | 0.176 | 0.076 0.185 9.375 pg/ml | 0.124 | 0.131 | 0.031 0.137 0 pg/ml | 0.095 | 0.1 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 69.3 | 3.4 | 4.8 Intra-assay | 2 | 20 | 151 | 5.3 | 3.5 Intra-assay | 3 | 20 | 419 | 8.8 | 2.1 Inter-assay | 1 | 20 | 70 | 2.9 | 4.1 Inter-assay | 2 | 20 | 152 | 4.5 | 3 Inter-assay | 3 | 20 | 424 | 10.4 | 2.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail