Human Cmrf35-Like Molecule 6, CD300C ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD300 is a glycoprotein family of cell surface molecules that regulate a diverse array of cellular processes via their triggering and inhibitory receptor functions. The CD300 family of myeloid immunoglobulin receptors includes activating (CD300b, CD300e) and inhibitory members (CD300a, CD300f), as well as CD300c and CD300d, whose function is uncertain.

Concentration | O.D. | Average | Corrected ------------------------------------------ 600 pg/ml | 2.818 | 2.723 | 2.591 2.628 300 pg/ml | 1.499 | 1.583 | 1.451 1.667 150 pg/ml | 0.842 | 0.89 | 0.758 0.939 75 pg/ml | 0.468 | 0.502 | 0.37 0.536 37.5 pg/ml | 0.321 | 0.328 | 0.196 0.335 18.75 pg/ml | 0.241 | 0.227 | 0.095 0.213 9.375 pg/ml | 0.188 | 0.183 | 0.051 0.179 0 pg/ml | 0.131 | 0.132 | 0 0.134

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 28.8 | 0.79 | 2.7 Intra-assay | 2 | 20 | 125 | 4.8 | 3.8 Intra-assay | 3 | 20 | 380 | 14.8 | 3.9 Inter-assay | 1 | 20 | 28.7 | 2.4 | 8.3 Inter-assay | 2 | 20 | 126 | 10.1 | 8 Inter-assay | 3 | 20 | 382 | 22.9 | 6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail