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Human Complement Factor H-Related Protein 2, CFHR2 ELISA Kit

Product Specifications

Background

CFHR2 belongs to the complement factor H protein family. The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (CFHR-1 to -5) . Members of the H-related protein family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. CFHR2 contains 4 Sushi (CCP/SCR) domains and is expressed by the liver and secreted in plasma. CFHR2 might be involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism.

Product Name Alternative

Complement factor H-related protein 2

Synonyms

CFHL2; FHR2; HFL3; CFHR2; DDESK59

Gene Name

CFHR2

Gene ID

3080

UniProt

P36980

Target

CFHR2

Field of Research

Signal transduction, Immunology

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 600 pg/ml | 2.78 | 2.859 | 2.764 2.937 300 pg/ml | 1.513 | 1.481 | 1.386 1.449 150 pg/ml | 0.769 | 0.822 | 0.727 0.874 75 pg/ml | 0.507 | 0.486 | 0.391 0.465 37.5 pg/ml | 0.304 | 0.29 | 0.195 0.276 18.75 pg/ml | 0.181 | 0.193 | 0.098 0.204 9.375 pg/ml | 0.142 | 0.14 | 0.045 0.137 0 pg/ml | 0.097 | 0.095 | 0 0.093

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

9.38-600 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 87.5 | 5.2 | 6 Intra-assay | 2 | 20 | 195 | 8.9 | 4.6 Intra-assay | 3 | 20 | 463 | 20.2 | 4.4 Inter-assay | 1 | 20 | 88.4 | 8.8 | 9.9 Inter-assay | 2 | 20 | 195 | 14.1 | 7.3 Inter-assay | 3 | 20 | 459 | 28.1 | 6.1

Sensitivity

2.64 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

30651

Available Sizes

Frequently Asked Questions

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