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Human Cd48 Antigen, CD48 ELISA Kit

Product Specifications

Background

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Product Name Alternative

CD48 antigen

Synonyms

BCM1; BLAST1; BLAST; Hcd48; MEM 102; SLAMF 2; TCT.1

Gene Name

CD48

Gene ID

962

UniProt

P09326

Target

CD48

Field of Research

Cancer, Immunology

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 600 pg/ml | 2.237 | 2.355 | 2.27 2.472 300 pg/ml | 1.251 | 1.276 | 1.191 1.301 150 pg/ml | 0.723 | 0.705 | 0.62 0.686 75 pg/ml | 0.384 | 0.409 | 0.324 0.434 37.5 pg/ml | 0.235 | 0.252 | 0.167 0.269 18.75 pg/ml | 0.171 | 0.167 | 0.082 0.163 9.375 pg/ml | 0.13 | 0.128 | 0.043 0.126 0 pg/ml | 0.08 | 0.085 | 0 0.09

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

9.38-600 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 73.3 | 1.6 | 2.1 Intra-assay | 2 | 20 | 193 | 5.1 | 2.6 Intra-assay | 3 | 20 | 386 | 12.3 | 3.2 Inter-assay | 1 | 20 | 73.4 | 4.6 | 6.3 Inter-assay | 2 | 20 | 195 | 9.9 | 5.1 Inter-assay | 3 | 20 | 382 | 23 | 6

Sensitivity

1.7 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

27683

Available Sizes

Frequently Asked Questions

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