Mouse Intercellular Adhesion Molecule 1, Icam1 ELISA Kit

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1 (integrin) . When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 560 pg/ml | 2.142 | 2.247 | 2.114 2.351 280 pg/ml | 1.324 | 1.297 | 1.164 1.27 140 pg/ml | 0.689 | 0.714 | 0.581 0.738 70 pg/ml | 0.452 | 0.435 | 0.302 0.418 35 pg/ml | 0.309 | 0.293 | 0.16 0.277 17.5 pg/ml | 0.244 | 0.24 | 0.107 0.236 8.75 pg/ml | 0.171 | 0.17 | 0.037 0.169 0 pg/ml | 0.136 | 0.133 | 0 0.13

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 58.3 | 4.6 | 7.8 Intra-assay | 2 | 20 | 182 | 9.1 | 5 Intra-assay | 3 | 20 | 396 | 13.4 | 3.4 Inter-assay | 1 | 20 | 59 | 5.2 | 8.8 Inter-assay | 2 | 20 | 182 | 12.6 | 6.9 Inter-assay | 3 | 20 | 392 | 23.7 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail