Human Interleukin-1 Receptor-Like 1, IL1RL1 ELISA Kit

IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interleukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage proinflammatory function. IL33 is a specific ligand of ST2L and induces the production of Th2 cytokines.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.415 | 2.34 | 2.251 2.265 250 pg/ml | 1.434 | 1.385 | 1.296 1.336 125 pg/ml | 0.769 | 0.773 | 0.684 0.777 62.5 pg/ml | 0.422 | 0.427 | 0.338 0.432 31.25 pg/ml | 0.262 | 0.258 | 0.169 0.254 15.625 pg/ml | 0.186 | 0.174 | 0.085 0.162 7.8125 pg/ml | 0.13 | 0.133 | 0.044 0.136 0 pg/ml | 0.084 | 0.089 | 0 0.094

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 52.5 | 2.7 | 5.2 Intra-assay | 2 | 20 | 194 | 6.1 | 3.2 Intra-assay | 3 | 20 | 397 | 22 | 5.5 Inter-assay | 1 | 20 | 52.3 | 1.8 | 3.4 Inter-assay | 2 | 20 | 195 | 15.8 | 8.1 Inter-assay | 3 | 20 | 395 | 13.4 | 3.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail