Human Interleukin-18 Receptor Accessory Protein, IL18RAP ELISA Kit

Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiation w218b), is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand-binding subunit of the IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. IL18RAP is required for the high-affinity binding of interleukin 18 (IL-18) to its receptor complex. IL18RAP together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Two putative isoforms of IL18RAP were detected and the ratios and total levels of these isoforms may contribute to the etiology of coeliac disease. IL18R1 and IL18RAP polymorphisms have been found associated with diseases such as schizophrenia, HSV1 seropositivity, and atopic asthma. Analysis of IL18R1 and IL18RAP SNPs in 5 European prospective cohorts suggests that the variability of these genes are unlikely to contribute to modulate the risk of CVD in European populations.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 3.217 | 3.094 | 2.964 2.971 250 pg/ml | 1.706 | 1.797 | 1.667 1.888 125 pg/ml | 0.987 | 1.018 | 0.888 1.049 62.5 pg/ml | 0.571 | 0.545 | 0.415 0.518 31.25 pg/ml | 0.346 | 0.351 | 0.221 0.356 15.625 pg/ml | 0.255 | 0.243 | 0.113 0.231 7.8125 pg/ml | 0.182 | 0.184 | 0.054 0.186 0 pg/ml | 0.131 | 0.13 | 0 0.128

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 21.9 | 1.9 | 8.8 Intra-assay | 2 | 20 | 99.4 | 3.7 | 3.7 Intra-assay | 3 | 20 | 321 | 15.3 | 4.8 Inter-assay | 1 | 20 | 22.1 | 0.7 | 3.1 Inter-assay | 2 | 20 | 98.7 | 8.6 | 8.7 Inter-assay | 3 | 20 | 324 | 14.7 | 4.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail