Human Immunoglobulin Alpha Fc Receptor, FCAR ELISA Kit

FCAR, also called FcαRI or CD89, is a type I transmembrane receptor for Fc region of IgA which is the most abundant immunoglobulin in mucosal areas but is only the second most common antibody isotype in serum. This receptor is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, especially phagocytes located in mucosal areas. Upon ligand IgA binding, FcαRI associates with the FcR γ signaling molecule bearing the immunoreceptor tyrosine-based activation motif (ITAM) through a unique charge-based mechanism and triggers multiple cell-mediated immune responses. It has been reported that Fc RI is a dual-function receptor that can mediate both inflammatory and anti-inflammatory responses depending on the type of interaction with its ligand. Sustained aggregation of FCAR results in activation of target-cell functions such as antigen presentation and cytokine release. In contrast, Monomeric targeting with serum IgA or with a variety of anti-FcαRI Fab fragments triggers an inhibitory response and additionally induces apoptosis. FcαRI thus play an fundamental role in preventing tumor development and growth, as well as in controlling inflammation.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 1.577 | 1.493 | 1.401 1.409 250 pg/ml | 0.932 | 0.934 | 0.842 0.935 125 pg/ml | 0.513 | 0.546 | 0.454 0.578 62.5 pg/ml | 0.319 | 0.326 | 0.234 0.333 31.25 pg/ml | 0.198 | 0.208 | 0.116 0.218 15.625 pg/ml | 0.154 | 0.151 | 0.059 0.148 7.8125 pg/ml | 0.111 | 0.119 | 0.027 0.127 0 pg/ml | 0.095 | 0.092 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 38.3 | 2.8 | 7.3 Intra-assay | 2 | 20 | 113 | 7.8 | 6.9 Intra-assay | 3 | 20 | 296 | 13.8 | 4.7 Inter-assay | 1 | 20 | 38.5 | 3.8 | 9.9 Inter-assay | 2 | 20 | 113 | 10.6 | 9.4 Inter-assay | 3 | 20 | 293 | 22.7 | 7.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail