Human Bh3-Interacting Domain Death Agonist, BID ELISA Kit

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.759 | 2.712 | 2.605 2.665 250 pg/ml | 1.364 | 1.435 | 1.328 1.506 125 pg/ml | 0.759 | 0.805 | 0.698 0.851 62.5 pg/ml | 0.448 | 0.455 | 0.348 0.461 31.25 pg/ml | 0.275 | 0.286 | 0.179 0.297 15.625 pg/ml | 0.204 | 0.195 | 0.088 0.186 7.8125 pg/ml | 0.149 | 0.145 | 0.038 0.14 0 pg/ml | 0.113 | 0.107 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 28.1 | 1.8 | 6.5 Intra-assay | 2 | 20 | 87.9 | 4 | 4.5 Intra-assay | 3 | 20 | 293 | 8.4 | 2.9 Inter-assay | 1 | 20 | 27.9 | 2.1 | 7.5 Inter-assay | 2 | 20 | 88.9 | 4.5 | 5 Inter-assay | 3 | 20 | 290 | 13.2 | 4.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail