Human Carbonic Anhydrase 12, CA12 ELISA Kit

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.012 | 2.077 | 1.947 2.142 250 pg/ml | 1.096 | 1.084 | 0.954 1.072 125 pg/ml | 0.595 | 0.597 | 0.467 0.599 62.5 pg/ml | 0.356 | 0.378 | 0.248 0.401 31.25 pg/ml | 0.249 | 0.254 | 0.124 0.259 15.625 pg/ml | 0.178 | 0.188 | 0.058 0.199 7.8125 pg/ml | 0.155 | 0.16 | 0.03 0.165 0 pg/ml | 0.136 | 0.13 | 0 0.124

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 62.5 | 1.6 | 2.6 Intra-assay | 2 | 20 | 145 | 4.8 | 3.3 Intra-assay | 3 | 20 | 346 | 11.6 | 3.4 Inter-assay | 1 | 20 | 63.1 | 5.2 | 8.3 Inter-assay | 2 | 20 | 144 | 9.8 | 6.8 Inter-assay | 3 | 20 | 348 | 14.9 | 4.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail