Human Signaling Lymphocytic Activation Molecule, SLAMF1 ELISA Kit

CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. The CD150 receptor is expressed on thymocytes, activated and memory T cells, B cells, platelets, natural killer T cells, and mature dendritic cells, and is also detected on tumor cells of Hodgkin's lymphoma (HL) and diffuse large B-cell lymphoma with an activated B cell phenotype. Additionally, it is the immune cell receptor for measles virus (MV) . As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of interferon γ (IFN-γ) in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.844 | 2.884 | 2.779 2.923 250 pg/ml | 1.58 | 1.517 | 1.412 1.454 125 pg/ml | 0.725 | 0.747 | 0.642 0.768 62.5 pg/ml | 0.422 | 0.443 | 0.338 0.463 31.25 pg/ml | 0.272 | 0.276 | 0.171 0.279 15.625 pg/ml | 0.19 | 0.184 | 0.079 0.177 7.8125 pg/ml | 0.144 | 0.144 | 0.039 0.143 0 pg/ml | 0.111 | 0.105 | 0 0.098

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 33.4 | 1.5 | 4.6 Intra-assay | 2 | 20 | 111 | 5.1 | 4.6 Intra-assay | 3 | 20 | 398 | 19 | 4.8 Inter-assay | 1 | 20 | 33.5 | 2.8 | 8.3 Inter-assay | 2 | 20 | 111 | 6 | 5.4 Inter-assay | 3 | 20 | 395 | 19.9 | 5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail