Human Tumor Necrosis Factor Receptor Superfamily Member 1A, TNFRSF1A ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD) . CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 1.886 | 1.988 | 1.893 2.091 250 pg/ml | 1.165 | 1.177 | 1.082 1.19 125 pg/ml | 0.68 | 0.681 | 0.586 0.682 62.5 pg/ml | 0.402 | 0.396 | 0.301 0.39 31.25 pg/ml | 0.259 | 0.257 | 0.162 0.255 15.625 pg/ml | 0.171 | 0.174 | 0.079 0.177 7.8125 pg/ml | 0.135 | 0.135 | 0.04 0.136 0 pg/ml | 0.094 | 0.095 | 0 0.097

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 61.7 | 2.8 | 4.5 Intra-assay | 2 | 20 | 144 | 6.3 | 4.3 Intra-assay | 3 | 20 | 359 | 19.3 | 5.4 Inter-assay | 1 | 20 | 61 | 2.6 | 4.3 Inter-assay | 2 | 20 | 143 | 6.1 | 4.3 Inter-assay | 3 | 20 | 363 | 18.8 | 5.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail