Human Interleukin-18 Receptor 1, IL18R1 ELISA Kit

Interleukin-18 receptor 1 (IL18R1) also known as CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein, and CD218a, is an interleukin receptor of the immunoglobulin superfamily. IL18R1 is found expressed in the lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from the brain, skeletal muscle, pancreas, and kidney. A high level of expression is found in Hodgkin disease cell lines. This receptor is specifically bound to interleukin 18 (IL18) and is essential for IL18 mediated signal transduction. IL18R1 contains 3 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. It is a single-pass type I membrane protein. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The increased expression of IL18R1 may contribute pathogenically to disease and is therefore a potential therapeutic target. The absence of a genetic association in the IL18R1 gene itself suggests regulation from other parts of the genome, or as part of the inflammatory cascade in multiple sclerosis without a prime genetic cause.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.122 | 2.112 | 1.984 2.101 250 pg/ml | 1.365 | 1.346 | 1.218 1.327 125 pg/ml | 0.899 | 0.844 | 0.716 0.789 62.5 pg/ml | 0.554 | 0.519 | 0.391 0.484 31.25 pg/ml | 0.316 | 0.331 | 0.203 0.346 15.625 pg/ml | 0.238 | 0.231 | 0.103 0.223 7.8125 pg/ml | 0.172 | 0.178 | 0.05 0.183 0 pg/ml | 0.123 | 0.128 | 0 0.132

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 24.6 | 0.83 | 3.4 Intra-assay | 2 | 20 | 70.2 | 2.1 | 3.1 Intra-assay | 3 | 20 | 334 | 13.9 | 4.2 Inter-assay | 1 | 20 | 24.8 | 3.4 | 13.5 Inter-assay | 2 | 20 | 69.9 | 8.3 | 11.8 Inter-assay | 3 | 20 | 335 | 35.5 | 10.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail