Human Brorin, VWC2 ELISA Kit

Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein that contains two VWFC domains. VWC2 / Brorin is a BMP antagonist that may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 1.669 | 1.662 | 1.564 1.656 250 pg/ml | 1.073 | 1.117 | 1.019 1.161 125 pg/ml | 0.734 | 0.719 | 0.621 0.704 62.5 pg/ml | 0.423 | 0.421 | 0.323 0.419 31.25 pg/ml | 0.276 | 0.261 | 0.163 0.246 15.625 pg/ml | 0.167 | 0.169 | 0.071 0.172 7.8125 pg/ml | 0.126 | 0.135 | 0.037 0.144 0 pg/ml | 0.092 | 0.098 | 0 0.105

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 36.6 | 1.7 | 4.7 Intra-assay | 2 | 20 | 96.4 | 3.4 | 3.5 Intra-assay | 3 | 20 | 301 | 6.4 | 2.1 Inter-assay | 1 | 20 | 36.5 | 1.5 | 4.2 Inter-assay | 2 | 20 | 96.6 | 2.8 | 2.8 Inter-assay | 3 | 20 | 301 | 7 | 2.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail