Human Insulin-Like Growth Factor-Binding Protein 6, IGFBP6 ELISA Kit

Insulin-like growth factor-binding protein 6 (IGFBP6) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. IGFBP6 is directly downregulated by the beta-catenin/TCF complex in desmoid tumors, and imply a role for the IGF axis in the proliferation of desmoid tumors. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.66 | 2.577 | 2.481 2.493 250 pg/ml | 1.463 | 1.392 | 1.296 1.32 125 pg/ml | 0.765 | 0.755 | 0.659 0.744 62.5 pg/ml | 0.445 | 0.422 | 0.326 0.398 31.25 pg/ml | 0.248 | 0.257 | 0.161 0.265 15.625 pg/ml | 0.175 | 0.177 | 0.081 0.178 7.8125 pg/ml | 0.135 | 0.133 | 0.037 0.13 0 pg/ml | 0.097 | 0.096 | 0 0.094

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 40.7 | 1.7 | 4.3 Intra-assay | 2 | 20 | 113 | 3.5 | 3.1 Intra-assay | 3 | 20 | 276 | 7.6 | 2.8 Inter-assay | 1 | 20 | 40.5 | 3.2 | 7.9 Inter-assay | 2 | 20 | 114 | 8.8 | 7.7 Inter-assay | 3 | 20 | 277 | 15.4 | 5.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail