Mouse Tissue Factor Pathway Inhibitor 2, Tfpi2 ELISA Kit

Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI) . It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.807 | 2.69 | 2.6 2.573 250 pg/ml | 1.591 | 1.506 | 1.416 1.421 125 pg/ml | 0.804 | 0.766 | 0.676 0.728 62.5 pg/ml | 0.45 | 0.425 | 0.335 0.401 31.25 pg/ml | 0.274 | 0.26 | 0.17 0.246 15.625 pg/ml | 0.178 | 0.175 | 0.085 0.172 7.8125 pg/ml | 0.123 | 0.131 | 0.041 0.139 0 pg/ml | 0.088 | 0.09 | 0 0.092

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 39.3 | 2 | 5.1 Intra-assay | 2 | 20 | 123 | 4.8 | 3.9 Intra-assay | 3 | 20 | 396 | 17.4 | 4.4 Inter-assay | 1 | 20 | 39.2 | 4.1 | 10.4 Inter-assay | 2 | 20 | 122 | 6.5 | 5.3 Inter-assay | 3 | 20 | 400 | 17.5 | 4.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail