Rat Lilra5 ELISA Kit
Product Specifications
Background
LILRA5 is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LILR are a family of receptors possessing extracellular immunoglobulin domains. They are also known as CD85, ILTs, and LIR, and can exert immunomodulatory effects on a wide range of immune cells. ILT-11 contains 2 Ig-like C2-type (immunoglobulin-like) domains. It can be detected n tissues of the hematopoietic system, including bone marrow, spleen, lymph node, and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses
Product Name Alternative
LILRA5
Synonyms
CD85; ILT11; LIR9
Target
LILRA5
Field of Research
Immunology
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.735 | 2.738 | 2.606 2.741 250 pg/ml | 1.575 | 1.636 | 1.504 1.697 125 pg/ml | 0.903 | 0.934 | 0.802 0.965 62.5 pg/ml | 0.521 | 0.529 | 0.397 0.537 31.25 pg/ml | 0.34 | 0.337 | 0.205 0.334 15.625 pg/ml | 0.227 | 0.23 | 0.098 0.233 7.8125 pg/ml | 0.171 | 0.18 | 0.048 0.188 0 pg/ml | 0.128 | 0.132 | 0 0.136
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
7.81-500 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 41.2 | 1.1 | 2.8 Intra-assay | 2 | 20 | 82.4 | 2.4 | 2.9 Intra-assay | 3 | 20 | 358 | 11.1 | 3.1 Inter-assay | 1 | 20 | 41.3 | 2.8 | 6.7 Inter-assay | 2 | 20 | 83.3 | 5 | 6 Inter-assay | 3 | 20 | 354 | 27.8 | 7.8
Sensitivity
2.53 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Rat
Available Sizes
Frequently Asked Questions
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