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Rat Lilra5 ELISA Kit

Product Specifications

Background

LILRA5 is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LILR are a family of receptors possessing extracellular immunoglobulin domains. They are also known as CD85, ILTs, and LIR, and can exert immunomodulatory effects on a wide range of immune cells. ILT-11 contains 2 Ig-like C2-type (immunoglobulin-like) domains. It can be detected n tissues of the hematopoietic system, including bone marrow, spleen, lymph node, and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses

Product Name Alternative

LILRA5

Synonyms

CD85; ILT11; LIR9

Target

LILRA5

Field of Research

Immunology

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 500 pg/ml | 2.735 | 2.738 | 2.606 2.741 250 pg/ml | 1.575 | 1.636 | 1.504 1.697 125 pg/ml | 0.903 | 0.934 | 0.802 0.965 62.5 pg/ml | 0.521 | 0.529 | 0.397 0.537 31.25 pg/ml | 0.34 | 0.337 | 0.205 0.334 15.625 pg/ml | 0.227 | 0.23 | 0.098 0.233 7.8125 pg/ml | 0.171 | 0.18 | 0.048 0.188 0 pg/ml | 0.128 | 0.132 | 0 0.136

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

7.81-500 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 41.2 | 1.1 | 2.8 Intra-assay | 2 | 20 | 82.4 | 2.4 | 2.9 Intra-assay | 3 | 20 | 358 | 11.1 | 3.1 Inter-assay | 1 | 20 | 41.3 | 2.8 | 6.7 Inter-assay | 2 | 20 | 83.3 | 5 | 6 Inter-assay | 3 | 20 | 354 | 27.8 | 7.8

Sensitivity

2.53 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Rat

Available Sizes

Frequently Asked Questions

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